Duff limped off at half-time of the 1-0 defeat to Stoke with a calf problem last night.
Bobby Zamora is also struggling with an injury, with less than a week to go for the clash with Atletico Madrid.
“I don’t know about Damien yet,” admitted manager Roy Hodgson.
“He took a kick a while ago and it is cramping up his muscle. Bobby stands a chance but he’s not making vast progress on a daily basis. He’s touch and go.”
