Cole aggravated the injury against Atletico Madrid last week and required a painkilling injection in order to feature in Sunday’s 1-0 win over Manchester United.
A spokesman for Chelsea confirmed: “Ashley Cole has not been selected in the England squad for their friendly against Brazil.
“He has a depressed fracture of the tibia, an injury first picked up in the Atletico Madrid home match last month.
“He aggravated the injury before the match against Manchester United and required an injection to play.
“However, he further injured it during the game and has therefore been ruled out of this week’s England squad.”
