Totti has been struggling with a thigh problem and will not recover in time to make the clash with Lazio this weekend.
Tottis sustained the problem in the 3-1 win over Atlanta earlier this month.
“After having a scan in the morning (Tuesday) at Trigoria, Francesco Totti continues his rehabilitation programme with physiotherapy and swimming exercise,” Roma announced on their official website.
“Over the next few days he will train separately in the gym.
“An ultrasound to evaluate the course is scheduled for next week.”